USING A SOCIOCULTURAL LENS TO EVALUATE CANCER SURVIVORSHIP IN BLACKS AND LATINX IN THE US

Abstract Background A sociocultural lens such as the PEN-3 Cultural Model offers an opportunity to better understand the extreme psychosocial, physical, emotional, and financial challenges of Black and Latinx cancer survivors in the community experience. By focusing on cultural identity, relationships and expectations, and cultural empowerment, the PEN-3 Cultural Model applied in this study aims to offer a unique insight into cancer survivorship in the Black and Latinx communities in the U.S. Method. A qualitative descriptive approach was used on a purposive sample of 46 Black and Latinx participants recruited. Each of the 19 participants who are cancer survivors including 7 Blacks and 13 Latinx (Mage = 62 years), was interviewed using a semi-structured interviewing style. We also conducted 5 focus group discussions with 27 participants, 2 Latinx groups and 3 Black groups (Mage = 61 years). The focus group participants identified as caregivers of cancer survivors. Results Thematic analysis resulted in the following themes: culture and dealing with cancer, conflict and control, health care access barriers, and rethinking survivorship in the community. The themes offer insight into sociocultural factors that influence the quality of life of cancer survivors and those in their communities. Conclusion To better understand and address issues of cancer health inequity in the country, the findings of this study will offer an opportunity to advance knowledge on sociocultural factors influencing cancer survivorship in Black and Latinx communities.

Type 2 diabetes (T2D) is a major public health concern in older adults.Physical activity (PA) and healthy eating (HE) are two major lifestyle intervention strategies in diabetes self-management for controlling glucose levels, preventing complications, and improving functionality and quality of life.This qualitative study aimed to explore perceived factors influencing PA and HE among predominately Latino older adults with T2D ((age=70.5±4.8;Latinos=73.3%).We conducted semi-structured interviews with 15 community dwelling older adults with T2D at the end of a 12-week pre-and post-study assessing self-monitoring on lifestyles and health outcomes.All interviews were recorded and transcribed.Two researchers analyzed the data independently using a combination of deductive and inductive thematical analysis.Codes were categorized into five themes based on the socio-ecological framework, and sub-themes were identified.The themes included five levels of factors: 1) individual (e.g., knowledge, self-efficacy, self-monitoring, perceived benefits and threats, health status,); 2) interpersonal (e.g., family, friend, health provider); 3) organizational (e.g., availability of physical activity equipment in senior apartments); 4) environment (e.g., culture norms); and 5) policy factors (e.g., health provider, retirement) Nearly 80% of all patients with heart failure (HF) are older adults (≥65 years of age) who are at high risk for frequent hospitalizations.Older adults with HF may delay in responses to pain that could trigger the burden of HF symptoms such as dyspnea due to the increased cardiovascular workload and oxygen demands.However, there is a concern that healthcare providers may be poorly prepared to assess and manage pain when reported by specific populations.This qualitative secondary analysis examined the design recommendations of HF providers for a comprehensive home-based pain assessment and management tool for older adults with HF.The study used in-depth interviews with 20 healthcare providers specializing in HF across different states.Participants provided various recommendations, including a checklist of questions about the patient's past experience with pain, their living environment, and any activities that trigger pain.While some participants preferred electronic tools, such as an mHealth app, for data collection and analysis, they acknowledged the limitations of using technology for older adults.The tool should be straightforward, easy to navigate, and involve patients in the design process to ensure their motivation and buy-in.The tool should also consider the caregiver's input.In conclusion, this study highlights the importance of a comprehensive assessment of pain symptoms and daily activities to provide personalized care for older adults with HF.The design recommendations provided by healthcare providers can aid in the development of a unique home-based pain assessment and management tool for older adults with HF.A sociocultural lens such as the PEN-3 Cultural Model offers an opportunity to better understand the extreme psychosocial, physical, emotional, and financial challenges of Black and Latinx cancer survivors in the community experience.By focusing on cultural identity, relationships and expectations, and cultural empowerment, the PEN-3 Cultural Model applied in this study aims to offer a unique insight into cancer survivorship in the Black and Latinx communities in the U.S. Method.A qualitative descriptive approach was used on a purposive sample of 46 Black and Latinx participants recruited.Each of the 19 participants who are cancer survivors including 7 Blacks and 13 Latinx (Mage = 62 years), was interviewed using a semistructured interviewing style.We also conducted 5 focus group discussions with 27 participants, 2 Latinx groups and 3 Black groups (Mage = 61 years).The focus group participants identified as caregivers of cancer survivors.
Results.Thematic analysis resulted in the following themes: culture and dealing with cancer, conflict and control, health care access barriers, and rethinking survivorship in the community.The themes offer insight into sociocultural factors that influence the quality of life of cancer survivors and those in their communities.

Conclusion.
To better understand and address issues of cancer health inequity in the country, the findings of this study will offer an opportunity to advance knowledge on sociocultural factors influencing cancer survivorship in Black and Latinx communities.

BS GSA AND AGE JOINT SYMPOSIUM
Chair: Blanka Rogina Co-Chair: Rozalyn Anderson Since 2020, BS GSA and American Aging Association (AGE) recognized the opportunity to present a joint session regularly at each organization's national meeting.Given our unique positions in the field of aging research, we recognize the opportunity to advance each other's missions and strengthen the ecosystem to advance translation of aging research into practice and policy.This symposium has been a resounding success and an ideal forum for early-stage investigators who seek to develop a program at the interface of basic and clinical research.

IMPACT OF MITOCHONDRIAL MALFUNCTION IN AGING IS MEDIATED BY IMPAIRED LYSOSOMES AND ENDOPLASMIC RETICULUM Nuno Raimundo, Penn State College of Medicine, Hershey, Pennsylvania, United States
The hallmarks of aging are well established, and include mitochondrial malfunction, deregulated nutrient sensing, loss of proteostasis, and senescence, among others.While these hallmarks are all part of the aging process, they have typically been studied independently.We have found that mitochondrial malfunction can affect lysosomal activity, impacting nutrient sensing, and our that the crosstalk between mitochondria and other organelles such as endoplasmic reticulum and lysosomes affects cellular senescence, proteostasis and intercellular communication.Chronic mitochondrial malfunction causes perturbations in the lysosomal membrane which affect the function of this organelle as well as the recruitment of folliculin (FLCN) to the lysosomes.
FLCN regulates the balance between anabolism (stimulating mTORC1) and catabolism (repressing AMPK).Increased recruitment of FLCN to the lysosomal membrane in cells subjected to chronic mitochondrial malfunction results in hyperactive mTORC1 and inhibition of AMPK signaling, thus aberrantly promoting anabolism under conditions that are not optimal.Furthermore, peroxisomal β-oxidation increases, and expression of proteasome subunits decreases, with consequent perturbation of proteostasis.Overall, the impact of chronic mitochondrial malfunction over lysosomes triggers multiple perturbations in organelles and organelle crosstalk that culminate in aberrant nutrient sensing, metabolic rewiring, proteostasis impairment, senescence and release of sterile inflammation-related proteins to the extracellular medium that denote alterations in intercellular communication.We will build on these findings to explore an overall picture of organelle homeostasis in aging and in age-related diseases.Senescent cell accumulation drives aging and related pathologies including metabolic disorders and neurodegeneration, and their selective elimination is a promising therapeutic approach to treat multiple diseases of aging in humans.However, developing drugs to eliminate senescent cells requires specific biomarkers to selectively identify and target these cells in vivo.In this study, we have performed a comprehensive and quantitative proteomic profiling of surface proteomes (surfaceomes) of senescent cells to identify potential senescence biomarker candidates in human tissues.We induced senescence using ionizing radiation in a variety of cell types including lung fibroblasts, monocytes, vascular smooth muscle cells, aortic endothelial cells and preadipocytes.Senescence was validated using a panel of canonical markers including reduced proliferation, increased senescence-associated β-galactosidase activity, and increased expression of p21, p16, IL6, and others.The cell surface proteome was enriched using an optimized surface biotinylation approach (Glyco-cell surface capture) followed by data-independent acquisition (DIA) LC-MS/MS analysis on a Q-Exactive HF Orbitrap mass spectrometer.Our analysis revealed novel cell surface targets that were further validated by flow cytometry and immunohistochemistry.These results provide clinically useful information for establishing biomarkers and therapeutic targets to aid in the translation of senescence-targeted therapies to treat age-associated diseases.

RECOMMENDATIONS REGARDING THE DESIGN OF PAIN ASSESSMENT AND MANAGEMENT TOOL FOR OLDER ADULTS WITH HEART FAILURE
. The factors in one level could influence the factors in another level.Most current lifestyle interventions focus on individual level, and multilevel lifestyle interventions to promote healthy lifestyles, and improve health, independence, and quality of life in older adults with T2D are warranted.The input of additional stakeholders such as family members and community staff need to be accounted when designing multilevel intervention in older adults with T2D.